Fig. 5. An allelic series of phan mutations. (A) Shoots and flowers of Phan⁺ (wild type), and three mutants grown at the semi-permissive temperature of 20°C. (B) The phan-250 mutant allele carries an uncharacterised insertion (grey triangle) within the 5'-UTR (unfilled box) and phan-249 contains an insertion of Tam2 (black triangle) in the coding region 3' to the conserved myb region (striped box). The phan-164 mutation carries an insertion of Tam 7 (grey triangle) 45 bp further downstream. The arrowheads labelled A, B and C denote the positions of primers used in the expression analysis shown in D. (C) Amino acid sequence differences between the protein products of the phan-164 and phan-249 alleles compared to wild type, as inferred from cDNA sequences. Phan sequences are shown in white type, and novel amino acids encoded by the transposons in black. (D) Phan mRNA expression in Phan⁺ (wild type) and plants homozygous for different phan mutant alleles, assayed by RT-PCR using primers A and B, or by 3'-RACE using primer C and an oligonucleotide complementary to the poly(A) tail.