Fig. 1. Requirement for kit function in regeneration melanocyte development in regenerating fins. Shown are stage 7 regenerates from (A) wild type, (B) temperature-sensitive kit mutant (kitj1e99) reared and regenerated constitutively at 25°C, (C) kitj1e99 fish reared at 33°C and shifted to 25°C upon amputation, (D) kitb5-null mutant, (E) kitj1e99 mutant reared and regenerated constitutively at 33°C and (F) kitj1e99 mutant reared at 25°C and shifted to 33°C upon amputation. Regeneration melanocytes are indicated by black arrows. Amputation planes are indicated by black arrowheads, with distal to the right of figures. In Figures 1-3, red lines and arrows represent growth at 33°C, blue lines and arrows represent growth at 25°C, and associated small black arrowheads represent time of amputation. Scale bars: 200 µm.