Fig. 4. Regulation of homeobox gene expression in AIY. (A) ttx-3prom::gfp expression is absent in ceh-10(gm58) animals. Homozygous ceh-10(gm58) offspring of ceh-10(gm58)/+; mgIs18 hermaphrodites arrest as L1 larvae and were identified based on their clr phenotype. Expression was never observed (n>50). (B) ceh-23::gfp expression is dependent on ttx-3. Adult ttx-3(mg158); kyIs5 were scored. Quantification is shown in Table 1. (C) ttx-3prom::gfp expression is unaffected in ceh-23(ms23) null mutant animals (n>50). Note the morphological integrity of the AIY neuron in terms of its cell position and axon morphology.