Fig. 2. Ocular phenotype of Otx1^-/-; Otx2^+/- neonatal mice. Frontal paraffin sections of wild-type (A,B) and Otx1^-/-; Otx2^+/- (C,D) neonatal mice were stained with Cresyl Violet to asses morphology. Images in A and C were taken from sections at equivalent axial levels. B and D are high magnification views of part of A and C, respectively. Note that in mutants both eyes show very similar alterations. The lens is rotated about 90° dorso-nasally, NR is folded toward the lens and fibres exit the eye in aberrant positions (arrows in B,D points to fibre tracts). RPE is totally absent but for a patch of pigmented tissue (arrowhead in D). The majority of the RPE has differentiated as a NR (open arrows in D). lv, lens vesicle; nr, neural retina; on, optic nerve; pe, retinal pigment epithelium; sc, sclera; T, tongue. Bar: A,C, 900 µm; B,D, 300 µm.