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Fig. 7. Lens development abnormalities in Otx-deficient mice. Adjacent sections through E10.5 (A-C) and E12.5 OC (D-I) of wild-type (A,D,G), Otx1+/-; Otx2+/- (B,E,H), and Otx1-/-; Otx2+/- (C,F,I) mice were immunostained with a polyclonal antiserum against {alpha}-A-crystallin (A-F) or hybridised with digoxigenin-labelled probe for Prox1 (G-I). Note that lens development is delayed and the overall size of the lens is reduced in Otx1-/-; Otx2+/- embryos (C,F,I). Lack of RPE differentiation is evident in Otx1+/-; Otx2+/- mice even in the presence of an apparently normal lens (arrowheads in E,H). L, lens; lp, lens placode; nr, neural retina; ol, outer layer. Bar: 200 µm (A-C), 300 µm (D-I).