Fig. 3. Lung phenotypes of Pitx2 allelic combinations. (A,B) Diagram of asymmetric lung branching pattern. (C,D) Whole-mount in situ hybridization with the sonic hedgehog probe in 9.0 dpc wild-type (C) and abc^null; abc^null (D) embryos. (E-I) Dorsal aspect of adult wild-type (E), neonatal abc^null; ab^hypoc (F), abc^null; ab (G), abc^null +/- (H) or oblique view of ab; ab (I). Ca, caudal lobe; Crl, cranial lobe; LL, left lung. (J-N) 11.5 dpc whole-mount in situ with probe for sonic hedgehog: wild-type (J), abc^null; abc^null (K), abc^null; ab^hypoc (L), abc^null; ab (M) and ab; ab (N). (O-R) 12.0 dpc whole-mount in situ with probe for bone morphogenetic protein 4: wild-type (O), abc^null; abc^null (P), abc^null; ab^hypoc (Q) and abc^null; ab (R).