Fig. 1. Subcellular distribution of Bves in the epicardium and mesenchyme. (A) A section through the developing atrioventricular junction stained with D033 shows the localization of Bves (red, Cy3) in the developing epicardium (epi) and delaminated vasculogenic mesenchyme. MF20 (green, Cy2) shows the position of the myocardium. (B) A higher power view shows Bves localization in the epicardium (B846) in the periphery of epithelial cells (arrows) while Bves appears as a spot in mesenchyme (arrowhead, B,E). (C-E) Bves (red) localization in epicardial epithelium is seen co-labeled with PAN-cadherin antibody (green). DAPI (blue) marks nuclei. Localization of Bves at the cell-cell boundaries coincided with cadherin (C,D, arrows). Nuclear co-localization shows the ‘spot’ is in a perinuclear region of the delaminated mesenchyme (E, F-H, arrowhead). (F-H) Bves-positive mesenchymes in the subepicardial space. Colocalization of Golgi 58K protein antibody (green) with Bves antibody (red) reveals the Bves-positive ‘spot’ is confined the perinuclear region (F,G are merged in H).