Fig. 3. Bves is an integral membrane protein. (A) Schematic representation of Bves protein structure shows the predicted structure of Bves and the relative positions of antibody epitopes. (B) TNT reactions were conducted in the presence and absence of dog pancreatic microsomes. Both full-length and truncated Bves (tm), which lacks the putative transmembrane domain, were used. The first lane shows that truncated Bves is produced and present in the lysate, but it is not captured in the microsomal fraction (lane 2). In contrast, full-length Bves, produced in the absence of microsomes (lane 3), is captured by microsomes (lane 4) and shows a slight increase in mobility. (C) Confocal analysis of Bves distribution in REC epithelium is presented. Analysis of the x-y axis shows the peripheral distribution of Bves. x-z axis analysis demonstrates that Bves is located in the lateral cell compartment. (D) Bves (red) and E-cadherin (green) staining show the colocalization (yellow) of the two proteins in the membrane.