Fig. 4. (Top) Protein levels in Arm-expressing embryos are not significantly higher than in wild-type embryos, but much lower than in zw3 or axin germline clones. The zw3 lane contains extracts from four embryos from zw3^M11-1; ArmGAL4/+ crossed to wild type. Half the embryos will receive a paternal wild-type copy of zw3, reducing the number of true mutant embryos per lane. The axin lane contains extracts from four embryos from axn germline clone crossed to axn/TM3, therefore half the embryos will receive a zygotic wild-type copy of axn reducing the number of true mutant embryos. However, though the axin and zw3 lanes mix mutant and non-mutant embryos, they still show a significant increase in endogenous Arm protein levels. The Arm lane contains extracts from four embryos where the 67.15 GAL4 driver was crossed to Arm, therefore all embryos express Arm. The wild-type lane contains extracts from four OreR embryos. All embryos were selected to be at similar stages (stage 11 to 12). (Middle) ß-tubulin was used as a loading control, and shows that all lanes were loaded equally. (Bottom) Arm bands were quantitated using NIH Image, and the results graphed. The units are arbitrary.