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Fig. 5. {Delta}Arm acts downstream of zw3, and is independent of the increased levels present in zw3 germline clones. Confocal microscope images of stage 9 embryos showing Arm protein (N2, green) and nuclei (Hoechst, blue). (A) A wild-type embryo, OreR. (B) zw3M11-1;ArmGAL4/+ crossed to wild type. The embryo was also stained with {alpha}-Sxl to separate out the females (not shown). (C) zw3M11-1; ArmGAL4/+ crossed to {Delta}Arm. The embryo was also stained with {alpha}-HA to find embryos expressing {Delta}Arm, and with {alpha}-Sxl to separate out the females (not shown).