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Fig. 6. Axin functions in retaining Arm in the cytoplasm. (A) Confocal microscope images of embryos showing endogenous Arm protein (N2, green) and nuclei (Hoechst, Blue). (A) axin germline clone embryos show very specific nuclear and plasma membrane localization, and a lack of cytoplasmic staining. (B) Wild-type embryo shown for comparison where diffuse staining is observed throughout the cell. (C) 67.15 crossed to {Delta}Arm, UAS-Axin. This embryo was also stained with {alpha}-HA to confirm expression of {Delta}Arm (not shown). Diffuse staining is observed throughout the cell with no obvious preference for the nucleus. (D) 67.15 crossed to {Delta}Arm alone shown for comparison. Nuclear localization of endogenous Arm is observed.