Fig. 5. Hedgehog signalling is required for oligodendrogenesis in the basal forebrain. Dissociated cells of mouse E10.5 ventral forebrain were cultured in defined medium for up to 8DIV in the presence or absence of the HH inhibitor cyclopamine. The cultures were immunolabelled with polyclonal anti-NG2. (A) Numerous NG2⁺ cells developed after 8DIV in the control. (B) Their development was strongly inhibited by cyclopamine. (C) The experiment was quantified by counting NG2⁺ cells in more than ten randomly selected fields (x63 microscope objective). Four independent experiments gave similar results, one of which is illustrated (mean ± s.d.).