Fig. 3. Lineage tracing of targeted injections. (A) Control embryo. (B) Embryo injected with Xdsh-D2 into the dorsal animal two blastomeres at the 16-cell stage. Embryo displays severe dorsal flexure. (C) Fluorescence view of embryo in B; GFP fluorescence is present in the dorsalmost tissues and absent from ventral tissues. (D) Embryo injected with Xdsh-D2 into the dorsal vegetal two blastomeres at the 16-cell stage, showing short stout morphology. (E) Fluorescence view of embryo in D; GFP fluorescence is present in the both dorsal and ventral tissues and is excluded from the neural tube.