Fig. 5. Phenotypic effects of EN-XMeis3^1-333. (A,B) Phenotypes characteristic of dpp-Gal4/UAS-EN-XMeis3^1-333 flies grown at 24°C. Ectopic eyes are frequently observed in the ventral head region (arrows). (C) An eye-antenna disc from a third instar larva stained with anti-ELAV antibody. Ectopic photoreceptors are present in the presumptive ventral head region (arrow). (D) A typical antenna-to-leg transformation. The a2 segment appears normal. The a3 segment is transformed distally to distal leg tissue, as suggested by the presence of bracted bristles. (E) The distal region of a deformed and truncated third leg. Bristle patterns suggest that these structures are of tibial (arrow) and pretarsal (arrowheads) identities.