Fig. 5. hb expression during early embryogenesis. Nomarski views of immunostaining (A,B,E,G,I,M) compared with DAPI staining (blue, C,D,F,H,J,L) reveal S. americana (A-I,L,M) and L. migratoria (J,K) hb localization from egg lay to condensation of cells to form the blastodisc. After egg lay, Hb protein is found in the posterior 15% or so of the egg (A), in a granular network on the surface of the egg (B). As nuclei begin to clump at the posterior end of the egg (C,D), Hb protein is still evident cytoplasmically (E, same field as D), but then enters nuclei of the posterior (G) region of the egg (matching DAPI shown in F). Nuclei in more lateral regions of the same egg (outside of the posterior 15% region) do not contain Hb protein (I, matching DAPI in H). Grasshopper hb transcript is first detectable in the condensed blastodisc but not extra-embryonically (K, matching DAPI in J). At about 34 hours AEL, the disc starts to become asymmetric (L, this embryos is not stained for Hb), and a short while later when gastrulation has begun, all embryonic nuclei still express hb at some level. Expression is not seen in extra-embryonic nuclei (M, arrows point towards the head lobes of the embryo).