Fig. 2. Western blot analysis of Fj expression in larval tissue. ConA-concentrated extracts from third instar larvae of wild-type flies compared with (A) fj mutant flies and (B) transgenic line P60 containing the pHS-fj⁺ transposon, with or without a 30 minute heat shock. The blots were stained with anti-FjC. All lanes in A and B contain extracts derived from equal numbers of larvae, but blot B was developed for a shorter period of time. The three forms of the Fj protein are indicated by lines to the left of the blots.