Fig. 1. Accumulation of GL2 transcripts during embryo development. Median longitudinal sections of embryos were hybridized to GL2 antisense (A-D) or sense (E-H) RNA probes labeled with digoxigenin-UTP. The low GL2 hybridization signal in D does not necessarily reflect a reduction in RNA abundance, because this in situ technique is less sensitive with sections from mature embryos (Y. L. and J. S., unpublished observations). (A,E) Globular stage embryos. Bar, 5 µm. (B,F) Heart stage embryos. Bar, 10 µm. (C,G) Torpedo stage embryos. Bar, 20 µm. (D,H) Mature embryos. Bar, 25 µm.