Fig. 4. Fkh is not properly downregulated at the larval-to-prepupal transition in 2Bc mutants of the BR-C. Expression of fkh in the salivary glands of three different 2Bc mutants, 2Bc¹, 2Bc² and 2Bc⁴, was assayed by RT-PCR as described in the legend to Fig. 2. In contrast to wild-type animals (compare Fig. 2), high levels of fkh mRNA are maintained in the salivary glands of mutant late-third instar larvae and early prepupae.