Fig. 6. Electrophoretic mobility gel shift assays demonstrate differences between different T box proteins to interact with different oligonucleotides. (A) Oligonucleotides used in electrophoretic gel mobility shift assays. Only one strand is shown and core motifs are boxed. Arrows indicate mutations in control oligonucleotides. Use of these oligonucleotides in electrophoretic gel mobility shift assays prevented binding (data not shown). (B) Band shift assay. Single-headed arrows indicate positions of high mobility complexes (red, Xbra; green: VegT, blue: Eomesodermin). Double-headed arrow indicates low mobility complexes.