Development -- Hashino et al. 128 (19): 3773 Figure IG2

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Fig. 2. Expression of GFR ${alpha}$ 1 and Ret proteins declines in ciliary ganglion neurons after target innervation. (A) Western blot characterization of anti-GFR ${alpha}$ 1, GFR ${alpha}$ 2 and Ret antibodies used in the present study. The GFR ${alpha}$ 2 antiserum generated in this study recognizes an approx. 55 kDa protein, equivalent to the estimated size of GFR ${alpha}$ 2, in E9 ciliary ganglion neurons. The anti-GFR ${alpha}$ 1 antibody recognizes two protein species of approx. 55 and 65 kDa, whereas the anti-Ret antibody recognizes a single protein of 170 kDa in ciliary ganglion neurons. (B) Western blot analysis for GFR ${alpha}$ 1, GFR ${alpha}$ 2, Ret, CNTFR ${alpha}$ and gp130 in ciliary ganglion lysates (left) or whole brain lysates (right) at different embryonic ages.