Fig. 1. Specificity of the antisera against Xenopus B-type cyclins. (A) Five coupled transcription-translation reactions were set up, programmed with no added DNA (-) or with the indicated cyclin plasmids. (B) Aliquots were analyzed by SDS-PAGE in sets of 5, transferred to nitrocellulose and immunoblotted separately with the indicated affinity-purified antisera. The volume of translation mix was identical in all lanes. (C) The sensitivity of the antisera against cyclins B1, B2 and B4 were tested against dilutions of the bacterial antigens, and quantitated by scanning the immunoblots.