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Fig. 5. Cytological characterisation of oocyte maturation (minutes after white spot formation are shown above each panel). (A) Images from a time lapse video of maturing oocytes. An uninjected ‘Control’ oocyte is compared with an ‘Antisense’ injected oocyte containing 75 ng ‘cyc8’ and 25 ng of anti B5-2 antisense oligonucleotides. (B) Spindles and chromosomes during MI. Microtubules are green, phosphorylated histone H3 red. (C) MII meiotic spindles of control, antisense- and sense-injected oocytes. (D) Meiotic spindles in CHX-treated oocytes. Scale bars, 10 µm.