
Fig. 8. Effects of indestructible cyclin B1 mRNA and CHX. (A) Experimental design; dm indicates mRNA encoding indestructible cyclin B1 (RXXL to AXXA mutant). (B) Images of MII-arrested oocytes, CHX-treated oocytes and oocytes that were injected with mRNA encoding indestructible cyclin B1 30 minutes before addition of CHX. (C) Immunoblots and histone H1 kinase assays of oocytes subjected to the indicated treatments.