Fig. 6. Comparison of Foxd3 and Slug misexpression in electroporated embryos. (A-C) Ectopic Foxd3 did not upregulate the premigratory crest markers, Slug (A), Cad-6B (B), and RhoB (C) at 24 hours (A,B) or 48 hours (C) after electroporation. (D-H) Ectopic Slug did not induce ectopic expression of RhoB (D) or HNK-1 (E), 48 hours after electroporation. Ectopic Slug-positive cells remained within the neural tube and the interneuron patterning remained normal as shown by Pax2 expression (F). (G) Alternate sections of immunohistochemical (G) and in situ (H) analyses showing ectopic Slug-positive cells (green) and Foxd3 mRNA, respectively. Ectopic expression of Slug did not upregulate Foxd3 expression (arrows), nor did it suppress the expression of endogenous Foxd3 within the ventral regions of the neural tube (arrowheads). (I) Co-electroporation of Foxd3 and Slug expression vectors showed no enhancement of cell migration in Foxd3-positive/Slug-positive cells (yellow), 24 hours after electroporation compared with cells expressing ectopic Foxd3 only (see Fig. 3D). fp, floor plate.