Fig. 7. Lack of effect on the Pnr protein in the amnioserosa. The LP1 line drives expression only in the amnioserosa, as indicated by the LP1/UAS-lacZ embryo shown on the top. (A) Confocal image of a doubly labelled embryo stained for pnr and Kruppel. (B,C) The green and red channels, indicate that although there are high levels of pnr in the amnioserosa (B) there is no effect on Kr expression (C). (D) Embryo of the same genotype doubly stained for pnr and dpp. The expansion of pnr expression to the amnioserosa does not modify dpp expression, which remains normal.