Fig. 2. Progressive loss of PV and then AD hypothalamus in Nodal pathway mutants. (A-F) Lateral views of hypothalamic gene expression (indicated bottom right) in 28 hpf brains of embryos of various genotypes (indicated top right). Arrowheads in C and D indicate remaining shh and nk2.1a expression in the hypothalamus of MZsqt embryos. The arrow in D indicates posterior diencephalic expression of emx2. e, eye; h, hypothalamus; t, telencephalon; zli, zona limitans intrathalamica.