Fig. 5. Limb abnormalities induced by a dHAND mutant protein lacking the C-terminal region. F₀ transgenic mouse embryos were sacrificed at E16.5 and limbs were stained for bone and cartilage. (A) A forelimb from an embryo harboring prx-155-217. This construct induced preaxial polydactyly, as well as truncation of the zeugopod (indicated by black arrowhead). (B) Detection of dHAND transcripts by in situ hybridization to a longitudinal section through the developing ulna of an E13.5 embryo. dHAND expression is detected in the chondrocytes and perichondrium (white arrowhead). Note highest levels of expression in the hyperplastic zone of the chondrocytes (white arrow). Ectopic digits are indicated with an asterisk.