Fig. 2. RhoA^N19 expression disrupts dorsal closure and ventral integrity. UAS-RhoA^N19 expression using either enGAL4 (A-D) or prdGAL4 (E,F), causes defects in the dorsal and ventral cuticle. All embryos exhibit anterior holes (arrowheads) and some sort of lateral scarring. Expression of RhoA^N19 (A,E), and co-expression with GMA (B) can result in the formation of a posteriorly located dorsal hole (arrows indicate extent of the hole). Alternatively embryos exhibit puckering at the dorsal midline (C,F; white arrows indicates dorsal midline) and ventral cuticular holes (D,F).