Fig. 4. Expression of nos in EH cells. (A) Adult fly carrying the EHups-GAL4 and UAS-nos-tub3'UTR transgenes displays the adolescent phenotype. The same phenotype is produced by the UAS-nos-tub:TCE transgene. (B) Confocal image of fixed larval CNS tissue with EH cells (arrow) revealed by direct GFP fluorescence. In this experiment, animals carry the EHups-GAL4, UAS-GFP and UAS-nos-tub3'UTR transgenes. GFP fluorescence and bright field image are visualized simultaneously for orientation (BL, brain lobe; VC, ventral nerve cord). (C) Confocal projection of larval CNS of an animal from experiment in B shown at high magnification to illustrate the EH cell processes. The morphology of the EH cells and their processes is not obviously different from that of animals carrying only the EHups-GAL4 and UAS-GFP transgenes (not shown).