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Fig. 3. P7.p and P8.p remain unfused in ref-2(mu218) males. (A,B) Unfused Pn.p cells are inferred from the presence of ventral protrusions (indicated by white arrows) in a lin-12(n137); him-5(e1467) worm. lin-12(n137) males (A) contain a gap in the ventral protrusion pattern that corresponds to the region where P7.p and P8.p have fused with hyp7. No gap in the protrusion pattern is present in lin-12(n137); ref-2(mu218) males (B). (C,D) Unfused Pn.p cells visualized by immunostaining animals with the MH27 monoclonal antibody. P(3-6).p and P(9-11).p remain unfused in wild-type males (C), while P(3-11).p remain unfused in ref-2(mu218) mutant males (D).