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Fig. 3. Molecular organisation of the unc-53 gene. (A) The exon-intron structure of the unc-53 gene. The different SL1 transpliced sites are indicated, as well as the regions deleted in alleles n152 and e2432. Alternatively spliced exons are in pink. The extent of the phage and several cosmid clones are shown. The structure of the different GFP and minigenes constructs is also presented. (B) Structure of UNC-53. UNC-53 contains a calponin homology domain (CH, light green; amino acids 11-109), two proline-rich SH3-binding motifs, (SH3 b, dark green; 487-495 and 537-545), two coiled-coil regions (CC, red; 890-923 and 1078-1113; predicted by COILS at http://www.ch.embnet.org) and an ATPases associated with diverse cellular activities domain (AAA, yellow; 1292-1425) that contains a NTP-binding motif (1300-1307). The positions of the two potential LKK motifs (green, 114-133; 1097-1116) are indicated. Arrowheads mark the positions corresponding to the start of the e2432 and n152 deletions. (C) Alignment of the CH domain (PF00307), SH3-, LKK and NTP-binding motifs (PS00017). The Genbank Accession numbers for all the sequences used are: CeUNC-53, AF504312; HsNAV2, AX009326; Hs{alpha}-actinin, P12814; GgDystrophin, P11533; MmSOS, Q62245; DmSOS, P26675; MmDynamin, P39053; RnPI3K-p85, Q63787; HsVillin, XP_010866; HsDematin, Q08495; CeThymosin, T32473.