Fig. 5. Rescue of mys synaptic structure defects by genetic manipulation of FAS2 expression. (A) Phenotypic rescue at the third instar muscle 4 NMJ. mys^b9 causes undergrowth, whereas mys^ts1 causes overgrowth. Other phenotypic characteristics include increased satellite boutons and irregular bouton size. Black arrowheads indicate type Ib boutons. Open arrowheads indicate type 1s boutons. Scale bar: 20µm. (B) Quantification of NMJ synaptic growth in mys and fas mutant animals. In all genotypes, type I boutons on a minimum of 20 muscle-12 NMJs in hemisegment A2 were quantified. Error bars indicate s.e.m. Asterisks indicate significance relative to wild type. Circles indicate significance relative to each genotype without manipulated FAS2 levels. (* or °, P<0.05; ** or °°, P<0.01; *** or °°°, P<0.001).