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Fig. 10. Restoration of wild-type phenotype by ectopic expression of DdCAD-1. cadA-12 cells were transfected with the cadA cDNA construct and transformants that expressed similar levels of DdCAD-1 (strains WT33 and WT94) were analyzed. (A) Protein blots showing the expression of DdCAD-1 (arrowhead) in transformants. (B) Cell cohesion was assayed at 4 hours for strains WT33 ({square}), WT94 ({blacksquare}), cadA-12 ({circ}) and KAX3 (broken line). (C) Protein blots showing changes in the level of gp80 expression during development. Equal amounts of proteins were loaded and the actin band of the Coomassie Blue stained gel is shown. (D) Cell cohesion assays were carried out at different stages of development for strains WT33 ({square}), KAX3 () and cadA-12 ({circ}).