Development -- Wong et al. 129 (16): 3839 Figure IG2

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Fig. 2. Loss of EDTA/EGTA-sensitive adhesion sites in cadA^– cells. Cells were developed in 17 mM phosphate buffer (pH 6.4) for 4 hours. Cell aggregates were dissociated mechanically and cell reassociation was assayed in the presence of 10 mM EDTA or 10 mM EGTA. (A) Reassociation of KAX3 in the presence of EDTA ( ${triangleup}$ ) or EGTA ( ${square}$ ), or in their absence ( ${circ}$ ). (B) Reassociation of cadA-10 cells in the presence of EDTA ( ${triangleup}$ ) or EGTA ( ${square}$ ), or in their absence (). (C) Effects of soluble DdCAD-1 and anti-DdCAD-1 Fab on the reassociation of cadA-10 cells. Mutant cells were allowed to reassociate in the absence () or presence ( ${triangledown}$ )of 50 µg/ml soluble DdCAD-1 or 50 µg/ml anti-DdCAD-1 Fab ( ${lozenge}$ ) or 20 mM carnitine ( ${blacktriangleup}$ ). Data represent mean±s.d. (n=3).