Fig. 6. Serial DML transplant. (A) Schematic outline of the serial lip experimental procedure. The example shown is embryo s-03 (Table 2). (B-D) Composite confocal images of whole-mount embryos labelled with anti-desmin antibody (red) and anti-quail antibody (green). (B) Primary host after reincubation. The operated segment is highlighted by the broken line that indicates the position of the quail DML prior to harvest for transplantation into the second host. DML harvest caused the apparent disruption to somite morphology. The operated somite developed normally with a clear boundary between host and donor myotome (asterisks); centrally aligned quail nuclei are evident in the medial myotome. Some quail cells are apparent in adjacent somites. This is an artefact resulting from the harvest of the quail DML. (C) The secondary host after overnight re-incubation following DML transplant. The operated somite myotome grew medially to a similar extent as its neighbours and quail cells are evident at the DML and within the medial myotome. The quail-derived myocytes are not running completely parallel to the body axis, although they still remain within the somitic boundaries. (D) A higher magnification of the secondary host somite showing individual quail nuclei in myocytes (arrows). (E) A composite image of fluorescent dye labelling of transplanted tissue. Dye-labelled fibres in the myotome of the secondary host resulting from donor DML dye injections of DiI and DiO prior to transplant in the primary and secondary hosts, respectively. Some DiI-labelled fibres (arrowheads) are evident medial to DiO-labelled fibres (arrows) with some overlap.