(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Slam protein is expressed in the blastoderm embryo. Lateral views of embryos stained with {alpha}-Slam, with accompanying transverse 2 µm sections. Dorsal upwards, anterior towards the left in whole mounts. Dorsal upwards in sections. (A) Pre-cellularization (arrow marks Slam staining at membrane, arrowhead marks Slam staining basal to the nuclei). (B) Mid-cellularization. (C) End cellularization. Note decreased expression on ventral side of section. (D) Beginning of gastrulation. Invaginating mesoderm has lost Slam staining while Slam is still present in the dorsal epithelium of the section. (E) Stage 10, end of gastrulation. (F,G) Peptide competition experiment. Slam antibody was pre-incubated with the peptide used to generate {alpha}-Slam (F) or a control Nanos peptide (G) before incubation with wild-type embryos.