Fig. 2. (A) Sequence analysis of the DYAD gene. The top panel is a box diagram representation of DYAD showing the positions of the exons. Arrowheads indicate positions of primers used for examination of expression by RT-PCR. The predicted sequence of the DYAD protein is given below, and the region showing similarity to MS1 is underlined. The putative nuclear localisation signal is in bold. The dyad mutation causes a frameshift at aa position 505. (B) Analysis of DYAD expression by RT-PCR. The left panel compares DYAD expression in leaves (Lea) and inflorescence (Inf) of wild type. The right panel examines expression of DYAD in the inflorescence of wild type (wt) and mutant (dy) plants.