Fig. 5. Recombinant ephrin-A5 promotes collateral formation for thalamic axons. Phase-contrast photomicrographs and schematic drawings of individual axons from thalamic neurons cultured on membrane substrates isolated from NIH3T3-vector cells (A-D) and NIH3T3-ephrin-A5 cells (E-H). In the presence of ephrin-A5 ligand, thalamic axons present a profuse branching, whereas only few branches form under control conditions. Scale bar: 10 µm.