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Fig. 2. Chromosomal localisation and effect on PEV of the N- and C-terminal parts of Su(var)3-7. (A) Polytene chromosomes from salivary glands of transgenic larvae expressing the HA:FL, HA:1-6 or HA:{Delta}7-Ct constructs. Staining with an anti-HA antibody detected with a Cy3-labelled secondary antibody (red) and DAPI (blue). HA:FL and HA:1-6 did not require heat-shock induction for detectable expression. (B) Banding pattern of polytene chromosomes. HA:FL was heat shocked to detect a banding pattern. This was not necessary for HA:1-6. Staining is as in A. (C) Effect on Heidi variegation of the different tagged constructs. Eyes of heat shocked males from crosses between w; Heidi/CyO females and yw/Y; P{y+, HA:Su(var)3-7} males. Corresponding eye pigment measurements, normalised to the control Heidi/+:Heidi/+ = 100±5.4; Heidi/+; HA:FL/+ = 49±4.5; Heidi/HA:1-6 = 94±6.4; Heidi/HA:{Delta}7-Ct = 240±13.