Fig. 1. wg expression and phenotypes of wild-type and mutant wing hinges. (A) Adult wing and third instar larval wing imaginal disc showing wg expression detected by antibody staining. Bars indicate the regions amplified in B-E. (B) wg expression detected by X-gal staining in adult wing and by anti-Wg antibody in the wing pouch. Red arrows indicate the inner ring (IR); black arrows indicate the corresponding region in the adult wing. The arrowhead indicates the outer ring (OR). (C-E) Mutant phenotypes of spd^fg (C), rn^2–2 (D) and nub² (E). In all cases the hinge is deleted and the expression of wg, which is detected by staining of antibody to Wg, in the IR is missing.