Fig. 3. vg is required to activate the expression of wg, rn and nub in the wing pouch. (A-C) vg83b27R. The expression of wg (A), rn (B) and nub (C) in the wing pouch is missing (arrows; see Fig. 1F,G for wild-type expression). The expression in notum and legs was not affected. Ectopic expression of vg is sufficient to activate rn and nub. (D-F) dpp-GAL4/UAS-vg. Patterns of expression of wg (D), rn (E) and nub (F) in the eye/antenna disc. Arrowheads indicate the dpp expression domain in the antenna. rn and nub are expressed in similar patterns in leg and antenna. In D-F, ventral is leftwards.