Fig. 5. Rn and Nub are required for wg expression in the IR. (A-B) rn^2-2 clones, revealed by the absence of GFP (green), cell autonomously remove wg expression (red) in the IR. Other domains of wg expressions are not affected. (C,D) nub¹ clones, revealed by the absence of GFP (green), also remove wg expression (red) in the IR. As it has previously been reported, wg expression in the wing margin is expanded in these clones (Neumann and Cohen, 1998). Arrows indicate the IR. wg expression is detected with antibody to Wg. Dorsal is upwards and anterior is leftwards.