Fig. 5. Differential effects of MoSix3, MoPax6 or MoSix3/MoPax6 co-injected embryos. Dorsal views of embryos at the six-somite stage, anterior is towards the left; injected morpholinos and molecular markers for in situ hybridization are indicated. (A,B) MoSix3 results in the loss of Pax2 expression. (C,E) Retinal Pax2 is still expressed even in more severely affected MoSix3/MoPax6 double morphants. (F,H,I,K) In MoSix3/MoPax6 double morphants retinal Pax6 but not Six3 expression is reduced at early somitogenesis. (D,G,J) Pax2, Six3 and Pax6 expression are not affected in Pax6 morphants. broken yellow line outlines optic vesicle in B,K. mb, midbrain.