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Fig. 6. Macroarray analysis and real time PCR of liver cDNAs from B4B2A5m and control mice. (A) Radioactive spots obtained for IGF1, ESP1, IGFBP1 and glucose-6-phosphate (G6P) isomerase from membranes hybridized with labeled cDNAs obtained from control (CTRL) and B4B2A5m livers. Results are normalized using global standardization with AtlasImage 1.5 software. (B) Graphs representing normalized fluorescence intensity (Rn) as a function of cycle number and showing threshold cycles (CT) for IGFBP1 and GAPDH transcripts amplicons.