Fig. 7. Regulatory actions of Hoxa5 on the IGFBP1 promoter expression in HepG2 and HuF cells. Cells were transfected with the IGFBP1 promoter linked to luciferase, with/without FKHR and/or Hoxa5. (A) in the HepG2 hepatoma cell line, Hoxa5 alone has no effect, but it inhibits the FKHR-dependent activation of the promoter. (B) In the HuF cells, Hoxa5 activates the IGFBP1 promoter, and it cooperates with FKHR to enhance its activity dramatically. Graphs illustrate the result of one representative experiment; each bar represents the mean and s.e.m. from triplicates.