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Fig. 3. Functional analysis of wild-type and Hoxa5–/– stomachs. (A) Postnatal ontogeny of pepsin activity in the stomach of wild-type (circles) and Hoxa5–/– (squares) mice at different time points. Pepsin activity in Hoxa5–/– mutants remained statistically lower (black squares) at P17 and P30 compared with wild-type samples. IF immunostaining (B,C) and silver staining (D,E) showed that reduced enzymatic activity correlated with a decrease in zymogenic cells (arrowheads) and enteroendocrine cells (arrows) in Hoxa5–/– mutants (C,E) compared with wild-type specimens (B,D). Some gastric units were deprived of zymogenic cells (asterisk). Scale bar: 100 µm.