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Fig. 2. Eve repressor domains function autonomously in embryos. (A-D) Expression patterns of a responder transgene (diagrammed at the top right) driven by the stripes 4+6, 1 and 5 enhancers and containing a Gal4-UAS sequence, visualized by in situ hybridization (to lacZ mRNA). (E-G) Expression patterns of the Gal4 fusion proteins indicated on the left, which contain Eve repressor regions, driven by the stripe 1 and 5 enhancers. The patterns in E and G were visualized by staining with polyclonal {alpha}-Eve antiserum, and that in F with monoclonal antibody 2B8, which specifically recognizes the Eve C terminus (see text), all in Df(2R)eve embryos. (A) The responder transgene alone, expressed in the pattern of eve stripes 1, 4, 5 and 6. Note that stripe 5 is slightly stronger than stripes 4 and 6. (B) The responder repressed by Gal4-Eve-RC, present in the stripe 1 and 5 regions, as shown in E (one copy of each transgene). Note that stripe 1 is dramatically reduced, while stripe 5 is slightly reduced relative to A. (C) The responder repressed by Gal4-Eve-C. Note that stripe 1 is strongly reduced, although not as much as in B, while stripe 5 is again slightly reduced. (D) The responder repressed by Gal4-Eve-R. Note that stripe 1 is clearly reduced, while stripe 5 may also be reduced.