Fig. 3. Pka-RII localization in Akap200 mutants. (A,B) Control germaria. Akap200 (A) and Pka-RII (B) localized to membranes of germline and follicle cells in the germarium (arrowhead in B). (C-H) Akap200 mutant ovaries. In Akap200^k07118/Df(2L)N22-14 germaria, most of the Akap200 immunoreactivity was lost (C), resulting in a decrease of Pka-RII membrane localization in germline cells (D, arrowhead). Females hemizygous for the excision allele Akap200^ix4/Df(2L)N22-14 exhibited a partial restoration of Akap200 immunoreactivity in the germarium (E). As a result, Pka-RII membrane association was partially restored (F, arrowhead). Females hemizygous for the strongest allele (Akap200⁷/Df(2L)N22-14) had almost undetectable Akap200 immunoreactivity (G) and decreased Pka-RII membrane localization (H, arrowhead). Pka-RII membrane localization was not completely eliminated by this allele, however. Note that the total amount of Pka-RII protein appeared to be unchanged in all Akap200 mutants; only the fraction associated with membranes and the fraction found in the cytoplasm changed.