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Fig. 5. Altering Akap200 expression levels changes ring canal size. (A) Confocal projection of wild-type stage-ten egg chamber stained with rhodamine phalloidin. (B) Similar staged Akap200k07118/Df(2L)N22-14 egg chamber. Note the larger, thinner ring canals. (C) Similar-staged nos-GAL4-VP16/UAS-Akap200 egg chamber. Females containing these transgenes overexpressed Akap200 throughout the germline, resulting in smaller, thicker ring canals. Insets show a representative ring canal from each egg chamber, magnified a further four times.