Fig. 5. Fz8a synergies with Wnt8b. The co-injection of fz8a (5 pg/embryo) with wnt8b (0.5 pg/embryo) causes radialised phenotype (A); however, co-injection of higher doses of fz8a (50 pg/embryo) with wnt1 (5 pg/embryo) does not induce radialised phenotype (B). Dominant-negative form of Fz8a (fz8a-CRDTM) rescues loss of eye phenotype driven by wnt8b overexpression (D-F). 25 hpf embryos from uninjected control (C), wnt8b injection (D) and wnt8b/fz8a-CRDTM co-injection (E,F). Embryos were injected with fz8a-CRDTM, followed by either treatment with LiCl (or not) at the shield stage, and then further cultured to 27 hpf (J). The anterior brain defects caused by wnt8b overexpression are rescued by fz8a-CRDTM (G-I,K-R). The expression of anf (telencephalon and diencephalon), rx3 (eye primordia) and six3 (eye primordia), which disappeared at the putative anterior brain (H,L,P) of the 100% epiboly stage embryos, is restored by the co-injection of fz8a-CRDTM (I, M,N,Q,R). Scale bar: 100 µm in F; 250 µm in R.